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heat-inactivated sheep serum (hiss)  (Millipore)


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    Structured Review

    Millipore heat-inactivated sheep serum (hiss)
    Heat Inactivated Sheep Serum (Hiss), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/heat+inactivated+sheep+serum/heat+inactivated+sheep+serum/bio_rxiv__2024__08__17__605585-263-5-11
    Average 90 stars, based on 1 article reviews
    heat-inactivated sheep serum (hiss) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivatives
    Article Snippet: .. For immunofluorescence, frozen sections were briefly washed with 0.1% TritonX-100/PBS (PBST) and blocked with 3% bovine serum albumin (#A7906; Sigma-Aldrich/Merck) and 0.1% heat inactivated sheep serum (S-2263; Sigma-Aldrich/Merck) in PBST at RT for 1 h. The blocked sections were incubated with primary antibodies diluted in the blocking solution at 4 °C overnight. .. After being washed with PBST, the sections were incubated with Alexa Fluor® 488 goat anti-rabbit IgG (H + L) (#A-11034; Thermo Fisher Scientific) at RT for 1 h. After an additional washing with PBST, the sections were mounted with VECTASHIELD Antifade Mounting Medium with DAPI (#H-1200; Vector Laboratories, Newark, CA).

    Incubation:

    Article Title: Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivatives
    Article Snippet: .. For immunofluorescence, frozen sections were briefly washed with 0.1% TritonX-100/PBS (PBST) and blocked with 3% bovine serum albumin (#A7906; Sigma-Aldrich/Merck) and 0.1% heat inactivated sheep serum (S-2263; Sigma-Aldrich/Merck) in PBST at RT for 1 h. The blocked sections were incubated with primary antibodies diluted in the blocking solution at 4 °C overnight. .. After being washed with PBST, the sections were incubated with Alexa Fluor® 488 goat anti-rabbit IgG (H + L) (#A-11034; Thermo Fisher Scientific) at RT for 1 h. After an additional washing with PBST, the sections were mounted with VECTASHIELD Antifade Mounting Medium with DAPI (#H-1200; Vector Laboratories, Newark, CA).

    Article Title: Eml5, a novel WD40 domain protein expressed in rat brain.
    Article Snippet: We have isolated a novel transcript with homology to the major microtubule-associated protein in dividing sea urchin embryos, EMAP.. The protein has a predicted MWoff180 kDa and we have named it Eml5 (EMAP-like protein 5, GenBank accession no. AY445136).. Eml5 contains 11 putative WD40 domains and 3 hydrophobic stretches of 43 aa, HELP domains, which have been suggested to be involved in microtubule binding.

    Article Title: The Firre locus produces a trans-acting RNA molecule that functions in hematopoiesis
    Article Snippet: .. Samples were washed in 1X maleic acid buffer with 0.1% Tween-20 (MBST) and then incubated in Roche Blocking Reagent (Roche, 1096176) with 10% heat inactivated sheep serum (Sigma, S2263) for 4 hours at room temperature. .. We used an anti-digoxigenin antibody (Roche, 11093274910) at 1:5000 and incubated the samples for 18 hours at 4°C.

    Article Title: Prediction of microRNAs affecting mRNA expression during retinal development
    Article Snippet: .. After incubation in blocking solution containing 1× MABT, 2% blocking reagent (Roche, Mannheim, Germany) and 20% heat inactivated sheep serum (Sigma-Aldrich, Poole, UK) for 2 hours at RT, anti-digoxygenin Fab fragments (Roche, Mannheim, Germany) were applied at 1:5,000 dilution overnight at RT. .. Detection staining was performed by incubation with the alkaline phosphatase chromogenic substrate 5-bromo-4-chloro-3-indolylphosphate/nitro-blue tetrazolium (BCIP/NBT) (Roche, Mannheim, Germany).

    Blocking Assay:

    Article Title: Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivatives
    Article Snippet: .. For immunofluorescence, frozen sections were briefly washed with 0.1% TritonX-100/PBS (PBST) and blocked with 3% bovine serum albumin (#A7906; Sigma-Aldrich/Merck) and 0.1% heat inactivated sheep serum (S-2263; Sigma-Aldrich/Merck) in PBST at RT for 1 h. The blocked sections were incubated with primary antibodies diluted in the blocking solution at 4 °C overnight. .. After being washed with PBST, the sections were incubated with Alexa Fluor® 488 goat anti-rabbit IgG (H + L) (#A-11034; Thermo Fisher Scientific) at RT for 1 h. After an additional washing with PBST, the sections were mounted with VECTASHIELD Antifade Mounting Medium with DAPI (#H-1200; Vector Laboratories, Newark, CA).

    Article Title: PTHrP targets HDAC4 and HDAC5 to repress chondrocyte hypertrophy
    Article Snippet: .. After washing sequentially with SSC (2× SSC at room temperature for 30 minutes, 2× SSC at 60°C for 1 hour, and then 0.2× SSC at 60°C for 1 hour), we blocked the slides at room temperature for 1 hour using 5% heat inactivated sheep serum (MilliporeSigma, S3772) and 5% Blocking Reagent (Roche, 11096176001) in 100 mM Tris (pH 7.5)/150 mM NaCl buffer. ..

    Article Title: The Firre locus produces a trans-acting RNA molecule that functions in hematopoiesis
    Article Snippet: .. Samples were washed in 1X maleic acid buffer with 0.1% Tween-20 (MBST) and then incubated in Roche Blocking Reagent (Roche, 1096176) with 10% heat inactivated sheep serum (Sigma, S2263) for 4 hours at room temperature. .. We used an anti-digoxigenin antibody (Roche, 11093274910) at 1:5000 and incubated the samples for 18 hours at 4°C.

    Article Title: Prediction of microRNAs affecting mRNA expression during retinal development
    Article Snippet: .. After incubation in blocking solution containing 1× MABT, 2% blocking reagent (Roche, Mannheim, Germany) and 20% heat inactivated sheep serum (Sigma-Aldrich, Poole, UK) for 2 hours at RT, anti-digoxygenin Fab fragments (Roche, Mannheim, Germany) were applied at 1:5,000 dilution overnight at RT. .. Detection staining was performed by incubation with the alkaline phosphatase chromogenic substrate 5-bromo-4-chloro-3-indolylphosphate/nitro-blue tetrazolium (BCIP/NBT) (Roche, Mannheim, Germany).

    Article Title: A genome-scale study of transcription factor expression in the branching mouse lung
    Article Snippet: .. Tissues were then washed in KTBT and blocked for 2 hours in 2% blocking solution (Roche) and 20% heat inactivated sheep serum (Sigma) in KTBT at room temperature. .. The tissues were then incubated overnight at 4°C in the primary antibody, AP conjugated α-DIG, Fab fragment (Roche) at a dilution of 1:2000 using 2% blocking solution and 20% heat inactivated sheep serum in KTBT.

    Hybridization:

    Article Title: Eml5, a novel WD40 domain protein expressed in rat brain.
    Article Snippet: We have isolated a novel transcript with homology to the major microtubule-associated protein in dividing sea urchin embryos, EMAP.. The protein has a predicted MWoff180 kDa and we have named it Eml5 (EMAP-like protein 5, GenBank accession no. AY445136).. Eml5 contains 11 putative WD40 domains and 3 hydrophobic stretches of 43 aa, HELP domains, which have been suggested to be involved in microtubule binding.



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